Photo – Illinois Soybean Association

This project is using genetic engineering to impede soybean cyst nematode (SCN) reproduction and survival. Researchers will explore several methods and strategies, such as using CRISPR gene editing which has been used to create sterile male mosquito populations, and test in a controlled laboratory setting to identify the most viable option(s).

Read below for a project update provided by Dr. Matthew Hudson.

“This fall, we successfully established a protocol for cultivating SCN in our growth chamber, ensuring a consistent and reliable supply of nematodes for experimental use. We have developed protocols for extracting nematodes at all distinct life cycle stages, which required optimizing protocols to account for environmental, genetic, and unknown factors influencing SCN development. Multiple trials were conducted to determine the optimal time points for collecting each developmental stage, particularly different stages of the juveniles and the males.

We are now ready to start our first RNA-Seq experiment, designed to analyze all stages of the SCN life cycle with an emphasis on male- and female-specific gene expression. This step aims to uncover candidate genes for potential functional studies and gene transfer, contributing to advancements in nematode management strategies. Meanwhile, we are also developing microscopy and microinjection techniques to deliver genes into the nematodes.”

To learn more about this new checkoff funded research study, visit the Field Advisor Research Hub here.